Journal: Micromachines

Article Title: Photocatalytic Nanofabrication and Intracellular Raman Imaging of Living Cells with Functionalized AFM Probes

doi: 10.3390/mi11050495

Figure Lengend Snippet: Experimental setups for ( a ) cell membrane perforation based on a highly localized TiO 2 -mediated photocatalytic oxidation, and ( b ) intracellular tip-enhanced Raman spectroscopy (TERS) imaging of molecular dynamics in living cells. ( c ) Optical microscopy image showing an example of insertion tests or intercellular imaging for a living HeLa cell conducted using the atomic force microscopy (AFM) system after the penetration of the cell membrane using a TiO 2 - or AgNP-functionalized AFM probe.

Article Snippet: Cell viability after cell membrane penetration was estimated with Calcein-AM (Dojindo Molecular Technologies, Kumamoto, Japan); this dye stains only living cells, and causes the emission of a strong green fluorescence.

Techniques: Membrane, Raman Spectroscopy, Imaging, Microscopy

Journal: Micromachines

Article Title: Photocatalytic Nanofabrication and Intracellular Raman Imaging of Living Cells with Functionalized AFM Probes

doi: 10.3390/mi11050495

Figure Lengend Snippet: Probability of cell membrane perforation and penetration force obtained with a TiO 2 -functionalized AFM probe under UV irradiation as a function of indentation speed. The vertical bars represent the standard deviation of the penetration force. Note that each point and error bar in the plot of the penetration force represent the mean values and their standard deviations, respectively.

Article Snippet: Cell viability after cell membrane penetration was estimated with Calcein-AM (Dojindo Molecular Technologies, Kumamoto, Japan); this dye stains only living cells, and causes the emission of a strong green fluorescence.

Techniques: Membrane, Irradiation, Standard Deviation

Journal: Micromachines

Article Title: Photocatalytic Nanofabrication and Intracellular Raman Imaging of Living Cells with Functionalized AFM Probes

doi: 10.3390/mi11050495

Figure Lengend Snippet: Effects of indentation speed on the probability of cell membrane perforation and the penetration force (mean values and their standard deviations).

Article Snippet: Cell viability after cell membrane penetration was estimated with Calcein-AM (Dojindo Molecular Technologies, Kumamoto, Japan); this dye stains only living cells, and causes the emission of a strong green fluorescence.

Techniques: Membrane

Journal: Micromachines

Article Title: Photocatalytic Nanofabrication and Intracellular Raman Imaging of Living Cells with Functionalized AFM Probes

doi: 10.3390/mi11050495

Figure Lengend Snippet: Evaluation of cell viability after cell membrane perforation. ( a ) Bright-field microscopy image and ( b ) fluorescence microscopy image of a living HeLa cell that had been stained with 8 µM Calcein-AM before cell membrane perforation. ( c ) Fluorescence microscopy image of the HeLa cell after the penetration of the cell membrane by a TiO 2 -functionalized AFM probe at an indentation speed of 300 nm/s under UV irradiation.

Article Snippet: Cell viability after cell membrane penetration was estimated with Calcein-AM (Dojindo Molecular Technologies, Kumamoto, Japan); this dye stains only living cells, and causes the emission of a strong green fluorescence.

Techniques: Membrane, Microscopy, Fluorescence, Staining, Irradiation

Journal: Micromachines

Article Title: Photocatalytic Nanofabrication and Intracellular Raman Imaging of Living Cells with Functionalized AFM Probes

doi: 10.3390/mi11050495

Figure Lengend Snippet: TERS imaging of molecular dynamics inside a living cell. ( a ) A series of TERS spectra obtained inside a HeLa cell at elapsed times ranging from 0 to 40 min after the penetration of its cell membrane (laser power: 50 mW, acquisition time: 10 s, cumulative number: 3). ( b ) Time-dependent changes in the intensities of the representative Raman peaks associated with glycogen (484 cm −1 ), DNA (793 cm −1 ) and proteins (1004 and 1604 cm −1 ).

Article Snippet: Cell viability after cell membrane penetration was estimated with Calcein-AM (Dojindo Molecular Technologies, Kumamoto, Japan); this dye stains only living cells, and causes the emission of a strong green fluorescence.

Techniques: Imaging, Membrane